EN 374 Chemicals & Microorganisms

Glove protection against the penetration of chemicals and microorganisms.

The standard EN 374 Chemicals & Microorganisms for protective gloves specifies the requirements for a glove to provide protection against penetration of chemicals and microorgamisms. Penetration (Non-molecular level) referring to the movement of a chemical or microorganism through the protective glove material, seams, holes.

The minimum liquid-proof section of the glove shall be at least equal to the minimum length of the gloves specified in EN420.

Penetration: The glove shall not leak water or air when tested according to penetration, EN374-2:2014.

Chemicals can negatively affect glove materials they come in contact with. 

EN374-4:2013 defines the level of degradation for each chemical. The degradation result, in percentage (%), shall be reported in the user instruction.

Chemical Protection pictogram


Permeation (Molecular Level) refers to the ability of a liquid to penetrate the glove with the potential to come in contact with skin.
The ‘chemical resistant’ glove pictogram displays a digit codes below it, which indicate the chemicals for which a breakthrough time of at least 30 minutes has been obtained.

The following are the parameters for the breakthrough time testing:

  • TYPE A – 30 minutes (level 2) against minimum 6 test chemicals
  • TYPE B – 30 minutes minimum against 3 chemicals
  • TYPE C – 10 minutes (level 1) against minimum 1 test chemical

All chemicals listed above shall be tested for permation.

Microorganism Protection pictogramTesting against micro-organisms is compulsory for all gloves. The gloves are tested to protect against bacteria and fungi, but also viruses if requested. 

The ‘Micro-organism’ pictogram can be displayed if the glove conforms to at least performance 2 in the penetration test.

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